Antiproliferative effect of essential oil of Artemisia mendozana in tumor cells B16F0.

DARIO FERNANDEZ; EMILIO LIZARRAGA; ELISA MILLAN; LUIS ALBERTO LÓPEZ

CABA

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

Sociedades Argentinas de Biociencias

The essential oil of ajenjo (EO) contains 28 compounds of which the higher concentrated are: artemisia alcohol 4.8%, α-thujone 5.1%, borneol 11.2% and bornyl acetate 43.7%. In this project the effect of EO was analyzed in the in vitro proliferation of B16F0 murine melanoma cells. For the assay the cells were cultured with vehicle DMSO (control) or 0.1- 0.25 μl / mL EO dissolved in DMSO for 72 h. The growth rate (GI) ± SE was calculated from 3 independent experiments. At 72 h of culture, the GI of the control was 6.37 ± 1.07 and with EO was: 4.57 ± 1.07 (A); 1.37 ± 0.18 (B) and 0.43 ± 0.03 (C) for 0.13, 0.2 and 0.25 μL / mL respectively. GI of B and C were significantly different to the control (p≤0.001). These results show that EO, at concentrations, is a powerful inhibitor of the in vitro proliferation of B16 F0 cells.