PROTEIC FACTORS INVOLVED IN ADHERENCE OF Pseudobutyrivibrio xylanivorans ON HUMAN COLONIC CANCER CELLS (SW480)

S. RUIZ; M.C. GIMENEZ; N. SOHAEFER; L. PEREYRA; D. GRILLI; G.N. ARENAS; A. GAIA

San Luis

Congreso; XXXV Reunión Anual de la Sociedad de Biología de Cuyo; 2017

Sociedad de Biología de Cuyo

Recently, our investigation team achieved to isolate Pseudobutyrivibrio xylanivorans from the rumen of Creole goats. This strain has shown a higher capacity to degrade hemicellulose of alfalfa hay compared to related butirivibrios species. Due to this, we started studying of the probiotic capacity of P. xylanivorans. Firstly, it was necessary to study the adherence of the bacteria to the digestive tract. As a main requirement of probiotic potencial selection, we started an in vitro analysis of the adherence capacity of P. xylanivorans using as a model human: colon cancer cells SW480, which have similar characteristics to the ruminal epithelium. Bacterial adherence involves several mechanism, including the presence of fimbriae, proteinaceous structures in the bacterial surface (highly developed in proteolytic ruminal bacteria), glucocalix (used by many cellulolytic ruminal bacteria) and lectins. Thus, we compared the bacterial adherence to the SW480 cells with a standard bacterial inoculum (MOI 200) against a inoculum submitted to two heat treatments, in order to denaturalize the proteinaceous structures of the bacterial surface: 80°C for 60 minutes and 100°C for 1 minute. The adherence of P. xylanivorans to the SW480 cells significantly decreased (p