IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
TESTICULAR EFFICIENCY ALTERATIONS GENERATED BY HIGH-FAT DIETS ARE REVERTED BY OLIVE OIL-ENRICHED DIETS
Autor/es:
FUNES A; CABRILLANA M E; BARAUMA A; SIMON LAYLA; LANCELLOTTI, TANIA E. SAEZ; CONTE MI; COLOMBO REGINA; MONCLUS MA; FORNES MW.
Lugar:
Merlo
Reunión:
Congreso; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2017
Institución organizadora:
Soc. Biol Cuyo
Resumen:
Hypercholesterolemia is associated with several adult chronic diseases. Meanwhile, Mediterranean diets are considered protectors against hypercholesterolemia. We have recently demonstrated that hypercholesterolemia is associated with sub/infertility in rabbits and that olive oil (OO)-enriched diets reverse that damage. The objective of this work was to evaluate the effects of hypercholesterolemia on testicular efficiency and theirs reversion by OO-enriched diet. We fed adult rabbits with commercial diet supplemented with 14% of fat (HCR). After 6 months, a group of HCR was fed with commercial diet supplemented with 7% of fat and 7% of OO (½ HCR + ½ OO) in order to reverse the hypercholesterolemia. Tubular efficiency was determined by counting the number of spermatogenic cells. Also we calculated proliferation (iep) and differentiation (ied) indexes, dividing the number of spermatozoa by the percentage of spermatogonia and spermatids respectively. Apoptosis was analyzed by TUNEL. Finally, serum testosterone levels were detected. We found lower testicular efficiency in HCR with a decrease in spermatozoa number, spermatogonia and spermatids percentages, alterations in iep and ied, increase in spermatocytes and in germ cells apoptosis. These results accord with a simil-arrest in spermatogenesis and are linked with reduced testosterone levels. On the other hand, OO diets reverse this damage increasing the spermatogenic flow with higher percentages of mature cells (spermatids and spermatozoa) at the expense of spermatogonia. In this group, apoptosis was lower and testosterone higher than HCR. In conclusion, high-fat diets could promote male fertility alterations by affecting proliferation during spermatogenesis, and OO could reverse this damage.