IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
BIOLOGICAL CHARACTERIZATION OF A Trypanosoma cruzi STRAIN ISOLATED FROM A PATIENT WITH CHAGAS REACTIVATION FROM MENDOZA, ARGENTINA
Autor/es:
ROMANO PS; MARTINEZ SANTIAGO JOSE
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017
Resumen:
Chagas disease is an endemic Latin American zoonosis caused by the parasite T. cruzi that affects almost 8 million people worldwide. Benznidazole (BNZ) and Nifurtimox are the only drugs available today. These treatments are highly-toxic and cause serious side effects. Most in vitro studies of T. cruzi strains have revealed different susceptibilities to BNZ, displaying a wide range of IC50 values. In this work we have studied the BNZ susceptibility and cellular tropism of a natural T. cruzi isolated from a patient from Mendoza (TcM) and compared with a laboratory strain originally isolated in Brazil (TcY). Isolation was carried out from a blood sample of an adult patient with Chagas reactivation due to immunosuppressive therapy. Samples with blood trypomastigotes were placed on cell monolayers to produce the infection and subsequent isolation in cell culture. To analyze BNZ susceptibility, the proliferation of both strains in the epimastigote form (in axenic culture) and amastigote form (in cell culture) were studied in vitro in the presence of increasing concentrations of BNZ (1, 2, 5, 12, 25, 50, 75, 100 µM) for 72 and 48 h respectively. Our results showed that epimastigotes and amastigotes of TcM strain displayed similar values of IC50 of BNZ (11 ± 0.6 µM and 11 ± 0.55 μM respectively) whereas IC50 for TcY were 33 ± 1.7 µM for epimastigotes and 19 ± 1 μM for amastigotes. We also performed infection assays in epithelial (Vero), fibroblast (MEF) and muscle (H9C2, HSkM) cell lines to characterize the tropism of this isolate. In contrast to TcY, TcM displayed high affinity for muscle cells. We concluded that TcM is more susceptible to BNZ than TcY and that exhibits a myotropic behavior. From these results and considering that drug studies are usually performed with laboratory strains of T. cruzi, we recommend revising the doses established for BNZ therapy of Chagas disease.