IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The mast cell as a therapeutic target in neuropathic pain
Autor/es:
PENISSI AB; MARIANI ML; COLL R; VARGAS PM
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias 2017; 2017
Resumen:
Mast cells are one of the most important cell types responsible for neurogenic inflammation, the main neurochemical substrate of neuropathic pain. These cells release biologically active mediators such as serotonin in response to the activation by immunological and non-immunological stimuli. Mast cell activation by proinflammatory neuropeptides, such as substance P (SP) and neurotensin (NT), is one of the main mechanisms involved in neurogenic inflammation.The present study was designed to examine the effects of a lactone isolated from Artemisia douglasiana Besser (dehydroleucodine) and a xanthanolide isolated from Xanthium cavanillesii Schouw (xanthatin) on mast cell activation induced by SP and NT.Peritoneal mast cells from male adult Wistar rats were purified in Percoll, preincubated in the presence of test lactones (dehydroleucodine or xanthatin) and then challenged with the mast cell secretagogues SP and NT. Concentration-response and kinetic studies of mast cell serotonin release evoked by SP and NT (measured by HPLC with electrochemical detection), and evaluation of mast cell viability and morphology by light microscopy, were carried out. For statistics, Type 1 variance (ANOVA-1) was performed, followed by Tukey-Kramer test. P