IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
1079) PEDF (PIGMENT EPITHELIUM DERIVED FACTOR) EXPRESION IN MEPC5 CELLS (MOUSE) AND IN MALE REPRODUCTIVE TRACT (WISTAR RATS) UNDER ANDROGEN REGULATION
Autor/es:
FORNES MW.; MONCLUS MA; CONTE MI
Lugar:
CABA
Reunión:
Congreso; Reunion conjunta de sociedades científicas; 2017
Institución organizadora:
SAIC y otras
Resumen:
Abstract: Pigment epithelium-derived factor (PEDF) expressionhas been described in many organs as showing neurotrophic, anti-angiogenic, anti-apoptotic, anti-inflammatory, anti-oxidant andpro-cell survival properties. However, references to its activity inthe male reproductive system are scarce, except in prostate cancerand the regulation of sperm conjugation in rat epididymis. We aimedto characterize the expression of PEDF in MEPC5 cells (mouseepididymal proximal caput cells) and in the male reproductive tractof Wistar rats and explore their hormonal regulation. We found thatPEDF is expressed in MEPC5 by Immunofluorescence and over theepididymis, prostate and seminal vesicles by immunohistochemistry,but notably not in the testes. These results agree with those obtainby semi cuantitative RT-PCR. Androgen dependence of PEDFexpression was evaluated by flutamide administration during 15days to Wistar Rats. PEDF expression diminished along the malereproductive tract. This decreased expression was reversed after30 days without flutamide administration. The epididymis is an essentialessentialorgan in sperm maturation-storage. The role of PEDF in thisphysiological process has not been fully elucidated. But consideringthat in other systems PEDF has anti-apoptotic, anti-oxidants andpro-cell survival properties, its expression along the epididymis maybe related to the protection of spermatozoa while they are stored.Keywords: PEDF, male reproductive tract, MEPC5 cells, androgens.