NORADRENALINE AND ANDROSTENEDIONE RELEASES IN EX VIVO SYSTEMS BY STIMULUS OF CATECHOLAMINE

SOSA Z.; FÓSCOLO M.; PALMADA N; CAVICCHIA J

LA PUNTA, SAN LUIS. ARGENTINA

Congreso; XXVI REUNION CIENTIFICA ANUAL SOCIEDAD DE BIOLOGIA DE CUYO.; 2009

SOCIEDAD DE BIOLOGIA DE CUYO. ARGENTINA

161. NORADRENALINE AND ANDROSTENEDIONE RELEASE IN EXVIVO SYSTEMS BY STIMULUS OF CATECHOLAMINE Sosa Z2, Fóscolo M1, Palmada N1, Cavicchia J1. 1Instituto de Histología y Embriología (HIEM) Fac. de Cs Médicas UNCuyo and 2LABIR, Facultad de QBF, UNSL. The autonomic nerve fibres converge to the testis along two major pathways, the superior spermatic nerve (SSN) and the inferior spermatic nerve (ISN). The first one arises to the testis from the superior mesenteric ganglia (SMG) whereas the ISN originates in the inferior mesenteric ganglia (IMG). The aim of the present report was to evaluate 1º androstenedione (A2) release after the addition of noradrenaline (NA) in the ganglionic compartment in the system SMG- SSN- Testis (T) and in the system IMG- ISN-T and 2º to evaluate, the NA liberation in the testis compartment using both systems.The organs were incubated in Krebs Ringer at 37°C. A2 release was measured by RIA and NA by HPLC. Student test was applied with a significance of p<0.05. A2 and NA release in the T compartment showed a significant increase (p<0,001) at all times compared with the control groups when NA was administered in the IMG. However when administered in the SMG A2 shows no change and NA decreases in the T (p<0.001).These results show that catecholamine stimulus in peripheral nervous system would be involved in the testis physiology across NA and A2 release via SSN and ISN. Supported by SECYT of U.N. Cuyo and CONICET Argentina.