GLUTAMIC ACID: LOCATION AND FUNCTIONAL STUDIES ....

SORIA M; GIULIANI F; CABRERA R; FORNES M W

Ciudad de La Punta, SL

Congreso; Reunión Anual Soc. Biol. Cuyo; 2009

Soc. Biol. Cuyo

159. GLUTAMIC ACID: LOCATION AND FUNCTIONAL STUDIES IN SPERM, SPERMATOGENESIS AND FALLOPIAN TUBE IN MOUSE Soria MG, Giuliani FA, Cabrera RJ, Fornés MW. IHEM - Facultad de Ciencias Médicas - UNCuyo. Mendoza. Email: msoria@fcm.uncu.edu.ar The Glutamic Acid (Glu) is well studied at the central nervous system (CNS), although Glu receptors (GluRc) and Glu functions have not been fully described in sperms or fallopian tubes (FT). We reported the presence of GluRc in sperm cell and Glu as a triggering molecule of acrosomal reaction (AR). Now GluRc location was also extending to FT and mouse’s testis. The GluRc was detected during the spermatogenesis in specific stadium related to acrosomal development. On the other hand, the same antibodies imuno stained the epithelium of FT according to sexual cycle. Again superfussion assays (SA) to establish the release – uptake of Glu was definitive demonstrated at the CNS, but not at the FT. In FT is important because is the physiological place for induction of AR. Recently, SA in the FT was positive. Moreover, these interchange had also a relationship with the stadium of the female sexual cycle. The uptake and release was different within the female cycle showing high uptake during diestrus and releasing at the estrus. Results indicate that sperm possesses GluRc – progressively expressed during spermatogenesis -, Glu promote AR and Glu is secreted and capture by FT - following the sexual cycle – to promote high level of Glu at the fertilization place and moment.