EFFECTS OF CASTRATION AND TESTOSTERONE REPLACEMENT ON THE LUNG HISTOARCHITECTURE

PEREZ CHACA V; BIAGGIO VS; CABALLERO N; GARAY P; VINCENTI A E; FORNES M W; GIMENEZ M S; GOMEZ N N

Ciudad de La Punta, SL

Congreso; Reunión anual Soc. Biol. Cuyo; 2009

Soc. Biol. Cuyo

127. EFFECTS OF CASTRATION AND TESTOSTERONE REPLACEMENT ON THE LUNG HISTOARCHITECTURE Pérez Chaca MV, Biaggio VS, Caballero N, Garay PG, Vincent A, Fornes M, Giménez MS, Gómez NN. UNSL. IMIBIO. E-mail: perezch@unsl.edu.ar There is strong evidence that oxidative stress plays a key role in the pathophysiology of several lung diseases. The presence of specific androgens and estrogens receptors in the lung implies that sex hormones play a physiological role in pulmonary function. The present study was designed to determine whether castration and androgen replacement result in changes in the lung histoarchitecture. Wistar male rats (200± 20 g) were separated in three groups: controls (Co, n:6), castrated (Ca, n:6), and castrated replaced with testosterone (Ca+T, n:6), 60 days after castrations. The lungs were processed for light microscopy, image analysis system (ImageJ) was used and oxidative stress biomarkers were measured. ANOVA was used for statistical analysis. The results indicate that castration significantly affected the antioxidant status, its evidenced by a significant increase lipid peroxidation in serum TBAR’S (mM/mg protein), Co: 0.7± 0,0003 and Ca: 1,6±0,0009 (p<0.05). Significant morphometrical changes were observed in Ca (expanded alveoli, accumulation of polymorphonuclear and inflammation), Ca + T showed alternated collapsed and expanded alveoli and edema. Morphometry analysis of expanded alveoli in arbitrary range showed Co: 0,3± 0,02 vs Ca: 0,7±0,1 (p<0,01) and Ca+T : 1,1±0,3 vs Co (p<0,05). These results indicate that castration modified the rat lung tissue and testosterone replacement after castration produces a partial recuperation.