IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
DIETARY OLIVE OIL IMPROVES SEXUAL GLANDS HISTOLOGICAL ALTERATIONS AND TESTICULAR CHOLESTEROL METABOLISM IN HYPERCHOLESTEROLEMIC RABBITS Miguel Walter
Autor/es:
FUNES ABI; SAEZ LANCELLOTI TANIA; COLOMBO REGINA; FORNES MIGUEL; SIMON, LAYLA
Reunión:
Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017
Resumen:
DIETARY OLIVE OIL IMPROVES TESTICULAR CHOLESTEROL METABOLISM ALTERATIONS IN HYPERCHOLESTEROLEMIC RABBITSFunes, A- Simón, L- Colombo, R- Saez Lancellotti, E- Monclus, M- López,M- Cabrillana, M- Conte, M- Barauna,A- Fornés M. LIAM (IHEM), FCM - UNCuyo, CCT CONICET-Mendoza CIUDA-UDA. Argentina. abikarenina@yahoo.com.arThe relationship between hypercholesterolemia and reduced male fertility has been reported previously, but the mechanism involved remains unknown. Hypercholesterolemic rabbits (HCR) were associated with deleterious changes in sperm cells and sperm membrane was overloaded with cholesterol. Olive Oil (OO) supplementation improved semen parameters affected by high fat diet. The increase in membrane cholesterol could be due to changes in intracellular metabolism of this lipid. Within molecular regulation, SREBPs (Sterol- Regulatory-Binding-Proteins) are essential proteins for biogenesis and membrane homeostasis. We investigate the intracellular pathway of cholesterol in rabbit liver and testis under different diets (control; 0.05% cholesterol and protected by 7% OO). We found that after 3 and 6 months of diet the expression of mRNA encoding SREBP-2 and their target molecules did not show significant changes between hypercholesterolemic (HC), control animals and animals protected with OO but shows a tendency to increase after 3 months of fat diet and decrease after 6 months of fat diet and with OO. At protein level no significant variations were observed at 3 and 6 months of diet. These data showed that the testicle is sensitive to the intake of saturated or unsaturated fats, modulating the expression of molecules involved in intracellular metabolism of cholesterol. These results suggest that the poor semen quality found in HCR may be related to changes in metabolic status and the molecular machinery of testis. So, we are facing a possible target in the protection mechanism by OO.Keywords: hypercholesterolemia, fertility, cholesterol, olive oilArea temática: Biología General, Celular y Molecular (BM)