IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
KCTD15 induce la formación de vacuolas gigantes en células de mamíferos
Autor/es:
ZARELLI VALERIA; COLOMBO, MARÍA ISABEL; LOPEZ DE ARMENTIA, MARÍA MILAGROS
Lugar:
San Luis
Reunión:
Congreso; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2017
Resumen:
Kctd15 belongs to the potassium channel tetramerization domain (KCTD) proteins that contain bric-a-brac, tramtrak and broad complex (BTB) domain. The BTB domain is a protein-protein interaction motif that is found throughout eukaryotes. Kctd15 homologs are found in different vertebrates, representing a well -conserved family. It has been demonstrated that Kctd15 affect Wnt signaling and AP-2 transcription factor function during embryonic development in zebrafish. The molecular mechanism of these processes remains unknown. When we overexpressed Kctd15 in mammalian cells we observed the formation of giant vacuoles. In order to characterize these structures we used endosomal markers, such as Rab5, Rab7, CD63 and lysosomal markers (Lysotraker). Preliminary results form our laboratory indicates that these vacuoles do not acquired Rab5, Rab7 neither CD63. Moreover, Lysotraker was not observed inside the vacuoles. Taken together, these results suggest that the vacuoles induced by Kctd15 are not endosomal compartments. However, we did observed accumulation of GFP protein inside the vacuole, indicating that the membrane of the vacuole is permeable. We consider that this particular phenomenon induced by KCTD15 overexpression may play a key role in the regulation of a biological process.