PEDF (pigment epithelium derived factor) expresion in mepc5 cells (mouse) and in male reproductive tract (wistar rats) under androgen regulation

SIMÓN L; SAEZ LANCELLOTTI TE; BARAUNA AA; TAGLE DELGADO M.G.; CABRILLANA M.E; COLOMBO R; MONCLUS MA; CONTE MI; FUNES A.K.; LOPEZ ME; FORNES MW

Buenos Aires

Congreso; Reunión Conjunta Sociedades de Biociencias 2017; 2017

SAIB-SAIC-SAFE-SAA-SAB-SAI-SAH-SAP-SAB-SAFIS

PEDF (PIGMENT EPITHELIUM DERIVED FACTOR) EXPRESION IN MEPC5 CELLS (MOUSE) AND IN MALE REPRODUCTIVE TRACT (WISTAR RATS) UNDER ANDROGEN REGULATIONConte MI, Tagle ME, Simón L, Funes A, Cabrillana ME, Saez Lancellotti TE, López ME, Colombo R, Barauna AA, Fornés MW and Monclus MA.Abstract: Pigment epithelium-derived factor (PEDF) expression has been described in many organs as showing neurotrophic, anti-angiogenic, anti-apoptotic, anti-inflammatory, anti-oxidant and pro-cell survival properties. However, references to its activity in the male reproductive system are scarce, except in prostate cancer and the regulation of sperm conjugation in rat epididymis. We aimed to characterize the expression of PEDF in MEPC5 cells (mouse epididymal proximal caput cells) and in the male reproductive tract of Wistar rats and explore their hormonal regulation. We found that PEDF is expressed in MEPC5 by Immunofluorescence and over the epididymis, prostate and seminal vesicles by immunohistochemistry, but notably not in the testes. These results agree with those obtain by semi cuantitative RT-PCR. Androgen dependence of PEDF expression was evaluated by flutamide administration during 15 days to Wistar Rats. PEDF expression diminished along the male reproductive tract. This decreased expression was reversed after 30 days without flutamide administration. The epididymis is an essential organ in sperm maturation-storage. The role of PEDF in this physiological process has not been fully elucidated. But considering that in other systems PEDF has anti-apoptotic, anti-oxidants and pro-cell survival properties, its expression along the epididymis may be related to the protection of spermatozoa while they are stored.Keywords: PEDF, male reproductive tract, MEPC5 cells, androgens..