α1,2-AP2 EXPRESSION IN THE DEVELOPING BRAIN OF SPONTANEOUSLY HYPERTENSIVE RAT

SOSA MA; TRONCOSO M,; ASENSIO J,; SELTZER, AM

Mar del Plata

Congreso; XXXII CONGRESO ANUAL SAN 2017; 2017

Sociedad Argentina de Investigación en Neurociencias

Offsprings of spontaneously hypertensive rats (SHR) suffer chronic hypoxia during gestation,they have low body weight at birth and impaired neurological development. Clathrin mediated endocytosis is a mechanism involved in neuronal signaling, integrity and homeostasis. We aimed to assess changes in the expression of a member of the endocytic pathway, the adaptor protein AP2, in the developing brain of SHR. AP2 subunits (α1 and α2) expression were evaluated by western blot in cerebellum (CB), motor cortex (MC) and hippocampus (HIP) from P5 to P30 in SHR and WKY rat pups. Proteins levels were analyzed in postnuclear (PN), membrane (MB) and cytosol (CYT) subcellular fractions. Our results showthat α2-AP2 expression increased gradually from P5 to P30 in PN and MB fractions of CB, MC and HIP in both groups of rats. On the contrary, α1-AP2 did not present significant changes during development. In the CYT of CB and MC, the expression of α1-AP2 increased gradually in both strains and α2-AP2 remained stable; interestingly, in the HIP α2 subunit increased with postnatal age. These results indicate that for both rat strains, the subcellular distribution of α2-AP2 is region specific and the pattern of the protein expression is equivalent in all brainareas studied, throughout postnatal development. We conclude that the delay in the neurological development observed in SHR might not be related to alterations in theneurotransmission mechanism, endocytosis-dependent.