IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
NOVEL FINDING OF A TESTICULAR PROTEIN IN KIDNEY DUCTS.
Autor/es:
CABRILLANA ME; FUNES AK; VINCENTI AE; SIMÓN L; SAEZ LANCELOTTI TE; MONCLUS MA; COLOMBO R; FORNÉS MW
Lugar:
Mendoza
Reunión:
Congreso; XXXI Annual Scientific Meeting CUYO BIOLOGY SOCIETY; 2016
Resumen:
During sperm maturation, tail structures are stabilized, through oxidation of thiol groups. It was proposed that sperm thiol oxidation is necessary for sperm motility. This oxidation occurs in many proteins of the sperm, like ODF1. ODF1 is a cytoskeleton protein which function in the sperm has not been clarified at all. ODF1 was described as a testi and sperm protein exclusively, and was involved in the development of the flagellum. The aim of this work was to evaluate the presence of this protein in other tissues. We applied western blott, RT-PCR, and inmunofluorescence techniques using an antibody against ODF1. Surprisingly, the strongest signal was found in the proteins obtained from marrow kidney and cells from collecting duct. Also some cortical ducts presented positive mark. Extracts from brain, liver, skin, and lung did not show positive signal to ODF1 antibody. By RT-PCR we identified the mARN of this protein in kidney and tesits extracts.This was the first time that ODF1 was described in other tissue. In the future we will analyze if ODF1 participates in the development of the primary cilium in collecting ducts.