Finding of a testicular protein in kidney ducts.

MONCLUS MA; COLOMBO R; FORNES MW; CABRILLANA M.E; FUNES A.K.; VINCENTI A.E; SIMÓN L; SAEZ LANCELLOTTI T.E.

Mar del Plata

Congreso; LXI Reunión de la Sociedad Argentina de Investigación Clínica (SAIC),; 2016

SAIC SAI SAFE NANOMEDAR AACYTAL

During sperm maturation, tail structures are stabilized, through oxidation of thiol groups. It was proposed that sperm thiol oxidation is necessary for sperm motility. This oxidation occurs in many sperm tail protein, as in ODF1. ODF1 is a cytoskeleton protein which function in the sperm has not been clarified at all. ODF1 was described as a testi and sperm protein exclusively, and was involved in the development of the flagellum. The aim of this work was to evaluate the presence of this protein in other tissues. We applied western blott and inmunofluorescence techniques using an antibody against ODF1. Surprisingly, the strongest signal was found in marrow kidney extracts and cells from collecting duct. Also some cortical ducts presented positive mark. Extracts from brain, liver, skin, and lung did not show positive signal to ODF1 antibody. This was the first time that ODF1 was described in other tissue. Now we are evaluating the presence of the mARN of this protein in the kidney. In the future we will analyze if ODF1 participate in the development of the primary cilium in collecting ducts.