IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
4-hydrpxy-3-(3-methyl-2-butenyl)- acetophenone (4-HMBA) inhibits proliferation of melanoma B16F0 cells
Autor/es:
FERNÁNDEZ D; LIZARRAGA, E; MILLÁN, MARÍA ELISA; LUIS A. LOPEZ
Lugar:
Córdoba
Reunión:
Congreso; LII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2016
Institución organizadora:
SAIB
Resumen:
4-HMBA is the main secondary metabolite from Senecio nutans, commonly known as chachacoma, a medicinal plant widely used in Andean traditional medicine; this compound exhibits antifungal and tripanocidal activities.We analyzed in vitro the effect of 4-HMBA on proliferation of mouse melanoma cells. B16F0 cells were cultured in presence of ethanol (vehicle) or 5.0-17.5 μg/mL of 4-HMBA dissolved in ethanol. The growing index (GI) ± SE in 3 independent experiments, were assayed from 0 to 72 h. At 72 h of culture, GI of vehicle treatment, was 6.3 ± 0.7 and of 4-HMBA treatments were: 4.9 ± 0.7 (A); 3.2 ± 0.8 (B) and 1.0 ± 0.3 (C) for 7.5; 15 and 17.5 μg/mL respectively. Both (B) and (C) GI were p≤0.001 vs. vehicle (0 μg/mL 4-HMBA). These results show that 4-HMBA generated a significant inhibition of proliferation in B16 F0 cell in concentration below to 20 μg/mL. Experiments are running out in our lab to evaluate the effect of this compound in B16FO cell experimental melanoma in mice.