IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Involvement of a Catecholaminergic Agent in the Inferior Mesenteric Ganglion on Androstenedione Release Using an ex vivo System.
Autor/es:
CAVICCHIA, J.C.; FÓSCOLO,M.; PALMADA,N.; SOSA, Z.
Lugar:
Mendoza, Argentina
Reunión:
Congreso; XXVI Reunión Científica Anual Sociedad de Biología de Cuyo.MENDOZA. ARGENTINA; 2008
Resumen:
Involvement of a catecholaminergic agent in the inferior mesenteric ganglion on androstenedione release using an ex vivo system.  Cavicchia JC, Fóscolo M R, Palmada N, Sosa Z1 .IHEM.Cuyo Medical School, Cuyo University, Mendoza and 1LABIR, San Luis University. jccavic@ fcm.uncu.edu.ar The neural influence on testicular physiology is exerted both, through hypothalamic factors that control adenohypophysial hormone release and via the sympathetic ganglia. The nervous fibres which arrive from the inferior mesenteric plexus (IMG) follow the inferior spermatic nerve (ISN) and penetrate the testis though the epididymis. The aim of the present experiments was to found whether the catecholaminergic agents, norepinephrine (NE), and alfa (phentolamine) or beta (propranolol) antagonists administered in the IMG could modify the release of androstenedione (A2) from the testis of an ex vivo system made up of the IMG and the testis interconnected by the ISN. The incubation system consists in a thermostatic bath in standard conditions. When NE was added to the IMG the A2 release was measured by RIA. Samples were obtained at standard times. The experimental group was obtained adding NE or the antagonists, phentolamine or propranolol at 10-6M concentration. The agonist decreases A2 when administered in the left IMG (p<0.001) but increase it when administered in the right ganglion (p<0.001). Propranolol, when administered in the left IMG decrease A2 (p<0.001) but phentolamine increases A2 (p<0.05). In conclusion 1) This ex-vivo system is an appropriate model to study catecholaminergic agent influences on hormonal release from the testis 2) The present behavior would indicate the presence of A2 receptors in the IMG.