IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
HEMIN MODIFY THE LRP-1 TRAFFICKING IN ERYTHROLEUKEMIA CELL
Autor/es:
GROSSO RA; COLOMBO MI; CHIABRANDO GA; SÁNCHEZ MC; FADER CM
Lugar:
La Serena
Reunión:
Workshop; Actualizations in Membrane Trafficking in Health and Desease; 2016
Institución organizadora:
EMBO
Resumen:
Hemin is a natural compound which stimulates hemoglobin synthesis and organelle clearance necessary in erythropoiesis. The transmembrane low density lipoprotein receptor related protein 1 (LRP1), is the scavenger for the hemin-hemopexine complex that allows its internalization. Autophagy is a lysosomal-degradative process leading to degradation of non-necessary organelles. Our aim is to elucidate the possible role of LRP1 in the hemin-induced autophagy. Our preliminary results have shown that Hemin or resveratrol (autophagy inductors) are able to induce LRP1 gene expression and increase the protein levels. Moreover, in hemin-stimulated K562 cells (erythroleukemia cells line), it was observed an increased colocalization of LRP1 structures with late endosomes, lysosomes and also with autophagosomes. This is a new finding in LRP1 trafficking being reported that the receptor is only associated with early endosomes. Interestingly, silencing of LRP1, hamper the hemin-stimulated autophagy in K562 and HeLa cells. Moreover, in cells incubated in presence of hemin, an additional band of was detected by Western blot in a time-dependent manner. The study of the molecular mechanisms that regulate the autophagy induction could provide important data to understand more deeply the mechanisms of proliferation and differentiation of red line cells, especially in hematologic diseases such as leukemia.