IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
FREELY AVAILABLE TOOL FOR AUTOMATED QUANTIFICATION OF STAINED CELLS MICROSCOPY IMAGES.
Autor/es:
MASONE, D.; DEL VELIZ, S.; GOJANOVICH, A. D.; UHART, M.; FRONTINI LÓPEZ, Y. R.; BUSTOS, D. M.
Lugar:
Mendoza
Reunión:
Congreso; XXXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2016
Institución organizadora:
Sociedad de Biologia de Cuyo
Resumen:
p { margin-bottom: 0.25cm; direction: ltr; color: rgb(0, 0, 10); line-height: 120%; text-align: left; }p.western { font-family: "Liberation Serif",serif; font-size: 12pt; }p.cjk { font-family: "Noto Sans CJK SC Regular"; font-size: 12pt; }p.ctl { font-family: "FreeSans"; font-size: 12pt; }a:link { }Theanalysis of microscopy images is a powerful research tool, especiallyif it is quantitative and automatic. Accurate quantification ofcolored structures in stained cells is a routine task for cellularbiologists. User dependent image manipulation steps are the mostcommon error source in many quantification tools. Others requireexpensive software or have been developed for unstained cellularstructures, which often leads to over- or sub- estimation. Here weaimed at developing a freely available tool for automatedquantification of colored structures in cells. We used Oil Red Ostained lipid droplets ?and DAPI stained nuclei- of murine 3T3-L1preadipocytes and human mesenchymal stem cells undergoingadipogenesis as models to develop our algorithm. We generated a fullyautomatic algorithm capable of analyzing hundreds of images and ofquantifying cells of different origins, nuclei, lipid droplets orother structures using the freely available Octave package. Ourmethod can be used on either standard light microscopy or fluorescentimages, to extract a bulk of biologically relevant information.Prospectively, the quantification method described in this work couldbe applied to static or time-lapse data, collected with a simplevisible light or fluorescence microscopy equipment. Also, it isperfectly applicable to screenings to elucidate the functions ofgenes at systems level by investigating the phenotypes of a largenumber of cells in culture. The algorithm and/or technicalsupport/collaboration are provided under request.