EFFECT OF BIOMIMETIC MATRICES IN OSTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS

RIVERO, G. ; UHART, M.; JOFRE, A. B.; BUSTOS, D. M.; FRONTINI LÓPEZ, Y. R.; ABRAHAM, G. A.

Mendoza

Congreso; XXXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2016

Sociedad de Biologia de Cuyo

p { margin-bottom: 0.25cm; direction: ltr; color: rgb(0, 0, 10); line-height: 120%; text-align: left; }p.western { font-family: "Calibri",serif; font-size: 11pt; }p.cjk { font-family: "Calibri"; font-size: 11pt; }p.ctl { font-size: 11pt; }a:link { }Mesenchymalstem cells (MSCs) accelerate osteointegration of bone grafts andimprove efficiency and uniformity in the formation of bone tissue.MSCs are isolated from easily available and abundant sources,providing a practical and clinically attractive approach in bonetissue regeneration. Our aim was to study the effect of nanofibrousbiomimetic matrices composed of poly-ε-caprolactone (PCL) andnanohidroxyapatite (nHA) on MSCs osteogenicdifferentiation.MSCs isolated from adipose tissue plastic surgeries samples weredeposited above biomimetic matrices of PCL/nHA in 10% FBSsupplemented DMEM. We evaluated adhesion, penetration anddifferentiation of these cells cultured over nanofibrous matrices. Toinduce osteoblast differentiation, we supplemented the culture mediumwith a cocktail containing 5% horse serum, 10 mM β-glycerophosphate,0,1µMdexamethasoneand 50µM2-phospho-L-ascorbicacid. Osteogenic differentiation was assessed by measuring alkalinephosphatase (ALP) activity, which increased over time and itsrelative activity was higher in drug cocktail treated cells. MSCswere capable to penetrate the PCL/nHA scaffolds as observed in crosssections analyzed by transmission electron microscopy. These resultsare our first steps on the transition from monolayer to3D-nanofibrous biomimetic scaffolds cultures.