Methylglyoxal levels increased in hypercholesterolemic rabbits.

FUNES ABI; BARBIERI, ALEJANDRO; CHANTARASINLAPIN, P; VEISAGA, M; SIMON, LAYLA; SAEZ LANCELLOTTI , ESTEFANIA; FORNES MIGUEL

Congreso; Annual Meeting of the American Society of Cell Biology; 2016

Methylglyoxal (MG) is a major advanced glycation end product (AGE) under physiological conditions but this system is found increased two to five fold in patient with diabetes, a chronic adult disease (ACD). It is proposed that an increment in glucemiae is related to MG. In others ACD, instead of glucose, serum cholesterol is the marker for specific adult diseases. To study ACD with hypercholesterolemia several animal models (translational medicine) were developed. Classic animal model to study effects of HC is rabbit feeding with grease diet. In this model, some biochemical parameters were increased -cholesterol, glucose, triglycerides- and we found that MG accompanied these increments. Moreover olive oil added to grease diet - which decrease serum cholesterol- also pushed down MG. After feeding adult rabbit (HCR) with rabbit commercial balanced diet plus 0.5 % of cholesterol during 3 months, serum cholesterol increased three fold at least (87 ± 12 mg/dl) over control buds (NCR, 25 ± 4 mg/dl, p < 0,001). Curiously, triglyceridemia showed a little increment (HCR: 125 ± 25 compared to NCR: 98 ± 14 mg/dl, statistically non significantly); similarly, glycaemia increased mildly (HCR: 128 ± 18 compared to NCR: 111 ± 21 mg/dl, statistically non significantly); but MG determinations demonstrated a notable increment after six months of diet (HCR: 385 ± 46 compared to NCR: 96 ± 11µg/dl, differences between 6 months of treatment and 0 months). A 50% reduction of the grease added to diet was accompanied to a decrease in all of these parameters included MG (Cholesterol 62 ± 14; Triglycerides 111 ± 19; Glicemia 127 ± 11 and MG 74 ± 9 µg/dl). Moreover addition of olive oil to this fat reduced diet - normally used as a protected diet - promoted that biochemical parameters recover the normal values. MG normally linked to serum glucose level could be also related to serum cholesterol and the addition of olive oil normalized both, cholesterol and MG.