IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
SCREENING FOR INHIBITORS WHICH REGULATE THE SPERM SERIN PROTEASES
Autor/es:
MARISA CLEMENTI; PAOLA VANINA BOARELLI; MARIA DE LOS ANGELES MONCLUS; MIGUEL WALTER FORNES; ANDREINA CESARI
Lugar:
MENDOZA - ARGENTINA
Reunión:
Jornada; SOCIEDAD DE BIOLOGIA DE CUYO. XXVI REUNION CIENTIFICA ANUAL 2008; 2008
Institución organizadora:
SOCIEDAD DE BIOLOGIA DE CUYO
Resumen:
The regulation of serin protease activity in spermatozoa was attributed
to inhibitors found in seminal plasma, synthesized in accessory
glands that play an important role on sperm capacitation. In
the present work, we sought for serine protease inhibitors in bovine
seminal plasma (BSP) and murine accessory glands. We obtained
protein extracts from BSP and accessory glands from CF-1
mice. Small molecules were selected by filtration membranes
(30kDa). The inhibitors were detected by reverse zymography and
quantified by their ability to reduce trypsin activity over azocasein,
considering as 100% the inhibitory activity of SBTI. BSP showed
the highest inhibitory activity (85%). Seminal vesicle (VS) and
prostate (P) had the highest inhibitory activity (98-96% respectively),
whereas epididymis (E) and testis (T) had a significantly
lower activity compared to the control (SBTI): 14,5 and 7%
(p<0,05), respectively. The reverse zymography developed 1 protein
below 30 kDa in each sample analysed and curiously, 1-2 bands
between 26-66 kDa in most of them, except the SV. In conclusion,
we detected and preliminary characterized serin protease inhibitors
in PSB and accessory glands that potentially regulate sperm
serine proteases. MS identification of these proteins will be the
next step.