IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
HYPERCHOLESTEROLEMIC AND MEDITERRANEAN DIETS: CONSEQUENCES ON SPERM PHYSIOLOGY
Autor/es:
PAOLA VANINA BOARELLI; TANIA EMILCE LANCELLOTTI; SEBASTIAN ESPINOLA; ANDRES HUALPA; MIGUEL WALTER FORNES
Lugar:
MENDOZA - ARGENTINA
Reunión:
Jornada; SOCIEDAD DE BIOLOGIA DE CUYO. XXVI REUNION CIENTIFICA ANUAL 2008; 2008
Institución organizadora:
SOCIEDAD DE BIOLOGIA DE CUYO
Resumen:
Hypercholesterolemia is a causal factor of atherosclerosis and “cellular” diseases. Modified diets might lead to cut down serum cholesterol and also modified cell membrane. We investigate the effects of hypercholesterolemic (HCd) and Mediterranean diet (olive oil supplemented, Md) on rabbit sperms (S). The HCd treatment (16% bovine fat/total intake) caused significant increase (p < 0.05) in total serum cholesterol (121.8 ± 5.3 vs. 20.4 ± 2.7 mg/dl; control). Concerning sperm cell, we found changes in subcellular lipid distribution in sperm heads (Filipin III), reduction in sperm motility (53.6 ± 5,9 % vs. 72.4 ± 3.4 %, control; p < 0.05) and the acrosome reaction (AR, Triple Stain Test) showed a 20% decrease (p < 0.05) compared to control conditions. Md treatment (14% olive oil /total intake) significantly improved (p < 0.05) both membrane integrity (HOS-T) and AR. Experimental diets did not cause significant changes in others parameters as animal weight (control: 3.9 ± 0.5; HCd: 3.7 ± 0.3; Md: 3.8 ± 0.5 kg), libido and semen physical characteristics. In conclusion, saturated fat consumption promotes an increase in cholesterol (serum and sperm head membrane, acrosomal region), and a decrease in sperm motility and AR. Md might be associated to an improvement in S quality.