IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
HAVE HYPERCHOLESTEROLEMIC AND MEDITERRANEAN DIETS CONSEQUENCES ON SPERM PHYSIOLOGY?
Autor/es:
PAOLA VANINA BOARELLI; TANIA EMILCE LANCELLOTTI; SEBASTIAN ESPINOLA; FLAVIA CERRUTTI; ANDRES HUALPA; MIGUEL WALTER FORNES
Lugar:
CORDOBA - ARGENTINA
Reunión:
Congreso; 44TH ANNUAL MEETING ARGENTINE SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY RESEARCH SAIB; 2008
Institución organizadora:
SAIB
Resumen:
Hypercholesterolemia is a causal factor of atherosclerosis and cellular
diseases. Modified diets might lead to cut down serum cholesterol
and also modified cell membrane. We investigate the effects
of hypercholesterolemic (HCd) and Mediterranean diet (olive
oil supplemented, Md) on rabbit sperms (S). The HCd treatment
(16% bovine fat/total intake) caused significant increase (p <
0.05) in total serum cholesterol (121.8 ± 5.3 vs. 20.4 ± 2.7 mg/dl;
control). Concerning sperm cell, we found changes in subcellular
lipid distribution in sperm heads (Filipin III), reduction in sperm
motility (53.6 ± 5,9% vs. 72.4 ± 3.4%, control; p < 0.05) and the
acrosome reaction (AR, Triple Stain Test) showed a 20% decrease
(p < 0.05) compared to control conditions. Md treatment (14% olive
oil /total intake) significantly improved (p < 0.05) both membrane
integrity (HOS-T) and AR. Experimental diets did not cause
significant changes in others parameters as animal weight (control:
3.9 ± 0.5; HCd: 3.7 ± 0.3; Md: 3.8 ± 0.5 kg), libido and semen
physical characteristics. In conclusion, saturated fat consumption
promotes an increase in cholesterol (serum and sperm head membrane,
acrosomal region), and a decrease in sperm motility and AR.
Md might be associated to an improvement in S quality.