Migration characteristics of peritubular myoid cells from SHR rats

BERTOLDI MV, LOPEZ LA

Carlos Paz, Cordoba

Congreso; XLIV Reunion Anual SAIB; 2008

Sociedad Argentina de Investigacion Bioquimica

Migration characteristics of peritubular myoid cells from SHR rats Bertoldi M V, and López L A IHEM-CONICET. Facultad de Cs Médicas. Universidad Nacional de Cuyo. Mendoza. Argentina. llopez@fcm.uncu.edu.ar Peritubular myoid cells (PMC) form a layer that surrounds the seminiferous tubules (ST) into the testis and these cells have characteristics of smooth muscle cells. Hypertension affects vascular smooth muscle cells (VSMC) proliferation and migration. In our laboratory we have seen that PMC from testis of SHR rat (hypertensive rat) are more proliferative than PMC from testis of WKY rat (control rat). We analyzed whether PMC migration is affected by hypertension. PMC from WKY and SHR rats were isolated and cultured in DMEM/F12 with 10% fetal bovine serum (FBS). Migration assays were performed by the scratch wound motility assay. PMC were seeded in 24-well plates and grew to confluence. After 24 h serum deprivation, PMC were incubated in 0.1% FBS with or without Angiotensin II (AngII) or Endothelin-1 (Et-1). Wound closure rates were analyzed by direct microscopic visualization at 0 and 24 h. The assay showed that the migration of CMP WKY was 96.01% ± 4.4 (% ± SEM) and 141.03% ± 10.34 with AngII and Et-1 respectively. While migration of PMC SHR was 140.5% ± 9.67 and 111.7% ± 5.69 with AngII and Et-1 respectively. These results indicate that PMC SHR respond to AngII in different way than PMC WKY. Also, PMC SHR has similar behavior to VMSC SHR showing that PMC, a kind of not vascular smooth cells, are affected by hypertension.