Dehydroleucodine arrests HeLa cells in G2 phase.

COSTANTINO VV, LOPEZ LA

Carlos Paz (Cordoba)

Congreso; XLIV Reunion Anual SAIB; 2008

Sociedad Argentina de Investigacion Bioquimica

DEHYDROLEUCODINE ARRESTS HeLa CELLS IN G2 PHASE. VV Costantino, LA López. Instituto de Histología y Embriología IHEM-CONICET. Facultad de Ciencias Médicas. U. N. Cuyo. Mendoza.   Dehydroleucodine (DhL) is a sesquiterpene lactone isolated from the medicinal herb Artemisia douglasiana. The cell cycle is a collection of ordered processes that results in the duplication of the cells. The sequential phases G0/G1, S, G2 and M represent the major cell cycle states. Previous results from our laboratory showed that DhL delays the proliferation of HeLa cells. Our goal was to analyze in HeLa cells the cell cycle phase in which DhL arrests HeLa cells using flow cytometry and also to determinate the expression of cyclin B by western blot. For this propose 50x104 cells in quiescence were stimulated with 10 % fetal bovine serum (FBS) with and without 4 µM DhL, 24 h  The analysis of cell cycle phases indicated that at 24 h of stimulation 4,6% ± 1,0 (% ± SEM) and 11,1% ± 2,2 of the cells were found in G2 phase in the control and treated respectively. The statistical analysis indicated significant difference between the control and treated cells (p # 0.05). The determination of cyclin B showed a reduction of the levels in treated cells. These results indicate that DhL induces accumulation of HeLa cells in G2 phase and inhibits the expression of cyclin B.