IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Ruminal microbiome of goats fed on fresh alfalfa forage
Autor/es:
GRILLI D; MRAZEK J; KOPECNY J; FLIEGEROVA K; PAEZ LAMA S; CERÓN M; SOSA MA; ARENAS N
Lugar:
Portoroz
Reunión:
Simposio; 9th International Symposium on Anaerobic Microbiology.; 2015
Institución organizadora:
Univ. Ljubljana, Biotechnical faculty, Animal Sci. dept.
Resumen:
Domestic goats (Capra hircus) possess diverse adaptations to harsh environments by having a greater capacity to control the rumen environment. The aim of work was to determine both the abundance and diversity of bacterial community characterizing rumen of goats for which the diet was shifted from alfalfa hay (AH) to fresh alfalfa hay (FAF). Four animals were fed on alfalfa hay (AH) diet for a period of 50 days, then their diet was shifted to fresh alfalfa (FAF) for another 20 days. The rumen fluid samples were regularly collected five hours after feeding and the bloat levels were monitored. Total bacterial DNA was isolated by bead-beating approach combined with the column filtration steps of the QIAamp DNA Stool Mini Kit. Bacterial diversities were than estimated by quantitative PCR and by high throughput sequencing on Ion Torrent PGM platform with data analysis by QIIME software package.Conclusions:?switching the diet from alfalfa hay to fresh alfalfa caused bloating of the goats with highest bloat level 20 days after the diet change?both qPCR and NGS methods showed changes in microbial composition after diet change?changing the diet to fresh alfalfa caused increase of ruminococcaceae, corynebacterium and uncultured clostridiales and numbers and decrease of prevotella, lachnospiraceae and uncultured bacteroidales?The ratio of the phyla Firmicutes/Bacteroidetes was higher in fresh alfalfa-fed animals than in hay-fed animals.?animals fed on fresh alfalfa exhibited lower microbial diversity than animals fed on hay