Proteínas epididimarias relacionadas con la maduración espermática

RENSETTTI DE; MONCLUS MA; ORTÍZ MALDONADO V; CABRILLANA M.E; VINCENTI AE; FORNÉS MW

Huerta Grande, Córdoba

Congreso; I Reunión Conjunta de Sociedades de Biología de la República Argentina; 2007

Sociedades de Biología de Argentina: Mendoza, Córdoba, Tucuman, Rosario y buenos Aires

EPIDYDIMAL FLUID PROTEINS RELATED TO THE SPERM CELLS MATURATION Rensetti DE, Monclus MA, Ortiz Maldonado V, Cabrillana ME, Vincenti AE y  Fornés MW. I.H.E.M. Facultad de Cs. Médicas, UNCuyo - CONICET Email: mfornes@fcm.uncu.edu.ar The epidydimal transit allows the maturation, protection and storage of the sperm cells until they are ejaculated. As part of these processes we verified the motility development and the sperm-association due to their interaction with the protein microenvironment that surrounds them, principally in the distal region (Tail) of the organ. By   successive chromatography separation different protein fractions of caudal epidydimal fluid were isolated from adult Wistar rat. Then we incubated caudal sperm cells with the same protein fractions in vitro, to verify its capacity to maintain sperm motility and re associate epidydimal sperm cells forming rosettes. We detected that concanavalin A affinity fractions protein - with a MW between 80/45 KDa - (Fraction Nº 2) is the most effective to re associate sperm cells into rosettes, while with minor to 40 KDa MW enriched proteins (Fraction Nº4) participate to maintain sperm cells motility. Future efforts will conduct to isolated specific proteins form these fractions involved in sperm rosettes assemble and motility acquisition.