ENRICHED DIET WITH OLIVE OIL IMPROVES THE TESTICULAR INEFFICIENCY INDUCED BY FAT DIETS IN HYPERCHOLESTEROLEMIC RABBITS

SIMÓN L; FUNES A.K.; BOARELLI P.V; CABRILLANA M.E; MONCLUS MA; SAEZ LANCELLOTTI TE; FORNÉS M.W

Estancia Grande San Luis

Congreso; XXXII Reunión Científica Anual Soc. Biología de Cuyo; 2014

Sociedad de Biología de Cuyo

It had been observed in hypercholesterolemic rabbits, sperm abnormalities such as decrease in the number of ejaculated sperm and increase in abnormal forms. These disorders are reversed with diets supplemented with olive oil. A defective spermiogenesis could be the cause of the abnormal shapes observable by transmission electron microscopy and immunofluorescence; and reduced efficiency in spermatogenesis is responsible for the low number, demonstrable by epithelium seminiferous´ differential cell counts. In order to make this work, 3 groups of adult male New Zealand rabbits were generated: control (C), fat (F) by adding 14% animal fat (w / w), and protected (P) supplemented half of the fat of F (7%) with olive oil (OO 7% v / w) after 6 months of being fed with fat (14%) to achieve the hypercholesterolemia´s reversion. Animals were sacrificed and studied its testicular tissue. Ultrastructure showed changes on head formation and sperm nucleus, these results were confirmed with immunofluorescence. Dividing the seminiferous tubule into a proliferative and a differentiation compartment, all cell types were counted. It was observed that groups F and P have a depression of the first compartment. Efficiency in group F is decreased, with less number of spermatids. Olive oil induces a recovery, showing a significant increase in the elongated spermatids´ number at expenses of spermatocytes and round spermatids. These values are consistent with those observed in seminal sperm counts. Results suggest that OO reverses a negative effect of high fat diet on testis