FLUTAMIDE AFECTS SERPINE 1F EXPRESION IN RAT TISSUES

MONCLUS M.A; LOPEZ ME; CONTE MI; PIÑIERO BT; BECERRA MV; FORNES MW

Éstancia Grande San Luis

Congreso; XXXII Reunión Científica Anual Soc. Biología de Cuyo; 2014

Sociedad de Biología de Cuyo

Epididymal sperm association is observed in many species of mammals. In rat, these structures are called rosettes and requires a complete maturation of the sperm.. In previous work our group isolated and identified Serpine1 F, a protein involved in this associations: in the epididymal fluid. To identify whether the expression of this protein depend on androgen, Flutamide, a known antiandrogenic agent, was previously administered to animals for 15 days according to previously established protocols in the literature. Using RT-PCR and Inmunohistochemistry, different tissues were analyzed for mRNA and Serpin 1F expression. In analyzed tissues from animals treated with flutamide, Serpine 1F expression was not detected while the expression was observed in control animals. These results suggest that Serpin 1F secretion and the formation of rosettes in the epididymal tail is an androgen dependent phenomena.