DIET-INDUCED OBESITY IN MALE MICE IS ASSOCIATED WITH METABOLIC AND HYSTOLOGICAL DISORDERS IN TESTIS

FUNES A, SIMÓN L, DELLA-VEDOVA C, GOMEZ-MEJIBA S, SÁEZ LANCELLOTTI E, RAMÍREZ DC, FORNÉS M.

San Luis

Congreso; XXXII Reunion Cientifica Anual de la Sociedad de Biologia de Cuyo; 2014

Sociedad Biologia de Cuyo

DIET-INDUCED OBESITY IN MALE MICE IS ASSOCIATED WITH METABOLIC AND HYSTOLOGICAL DISORDERS IN TESTIS Funes A, Simón L, Della-Vedova C, Gomez-Mejiba S, Sáez Lancellotti E, Ramírez DC, Fornés M. LIAM (IHEM), FCM - UNCuyo, CCT CONICET-Mendoza and Laboratorio de Medicina Experimental & Terapéuticas, IMIBIOSLCONICET- UNSL,SanLuis.Argentina.E-mail: mfornes@fcm.uncu.edu.ar Overweigh and obesity are known causes of infertility in men, but the mechanism involved remains unknown. The aim of the present study was to investigate both reproductive and metabolic features of C57BL/6 mice fed a normal chow (LFD, low fat diet) or a 60% HFD (high-fat diet). At the end of the experiment (18 weeks), both reproductive and metabolic characteristics were assessed. Our data show that HFD caused significant metabolic alterations in mice, including obesity, glucose intolerance, dyslipidemia, and subtle liver steatosis. These findings suggest that HFD induces metabolic features in mice. Moreover, HFD showed differences in the expression of RNA messenger encoding proteins related to the synthesis and metabolism of cholesterol (SREBP-1, SREBP-2 and HMGCoAreductase). Few changes were observed histologically, but marked variations at the molecular level by comparing mice with control diet and fat. These data suggest that the abnormalities foundin spermatogenesis may be caused by changes in metabolic status and the molecular machinery of testis. EN PRENSA