Effects of melatonin on mast cell activation

SANTIANO F; GAURÓN MC; GIMÉNEZ MC; RECALDE GGM; MARIANI ML; PENISSI AB

San Luis

Congreso; XXXII Reunión Anual de la Sociedad de Biología de Cuyo; 2014

Sociedad de Biología de Cuyo

Melatonin is a chronobiotic hormone widely distributed in the body. It has a variety of extrapineal non chronobiotic functions such as neuromodulator action, antiproliferative, antioxidant, immunomodulatory and oncostatic. Immunomodulatory effects are exerted on different immune cells. However, the effect of melatonin on mast cell activation is unknown. The aim of this study was to determine whether melatonin inhibits mast cell activation induced by mast cell secretagogues that act by different molecular mechanisms of action.. Peritoneal mast cells from adult male rats were removed and then activated with compound 48/80, calcium ionophore A23187 and neurotensin. Morphological studies by light microscopy and confocal fluorescence microscopy were performed. The percentage of serotonin release was also determined by HPLC as a marker of degranulation. Melatonin inhibited mast cell activation induced by the calcium ionophore A23187 (10 µg/ml A23187: 16.35±1.6% versus 20 µg/ml melatonin: 2.8±0.21%, P