Antioxidants defenses involved in the tolerance to hibernation of a freshwater snail

GIUFFRIDA, PABLO M.; VEGA, ISRAEL A.; GIRAUD BILLOUD, MAXIMILIANO.

Buenos Aires

Congreso; VIII Meeting of the Society for Free Radical Biology and Medicine-South American Group (VIII SFRBM-SAG); 2013

SFRBM-SAG

Pomacea canaliculata is an invasive snail with effective mechanisms against lack water and extreme environmental temperatures. We have studied lipid peroxidation (TBARs), total antioxidant potential, and enzymatic (SOD) and non-enzymatic (GSH and uric acid/allantoin) antioxidants in control snails, snails after 45 days of hibernation, and aroused snails 30 min and 24h after RT water exposure, in hepatopancreas, kidney and foot. Both hepatopancreas and kidney showed a TBARs increase in hibernation, decreasing after arousal. Antioxidant potential of hepatopancreas was higher in hibernation and arousal than in control group, while in kidney the highest levels were observed in arousal groups. SOD activity UA was constant in all hepatopancreas groups, but GSH decreased in 30 min aroused animals. The kidney SOD activity decreased only in the early arousal group; in foot an increase of activity occurs during hibernation and early arousal. GSH decreased in hibernation and early arousal in kidney and in both foot arousal groups. Kidney UA increased during hibernation and decreased after arousal, while in foot the lowest levels were observed in hibernation and early arousal groups. Allantoin, the oxidation product of uric acid, remained constant in almost groups, except in hepatopancreas, where a significantly increase occurs in hibernation and 24h arousal. These results indicate that SOD, GSH and UA could protect different tissues during hibernation/arousal cycle.