IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ROLE OF -SNAP IN THE MAINTENANCE OF NCADHERIN- BASED ADHERENS JUNCTIONS IN MOUSE OVARIAN FOLLICLE
Autor/es:
ARCOS, ALEXIS; TORO, GABRIELA; GIANETTI D; DE PAOLA, MARIA MATILDE; MICHAUT, MARCELA ALEJANDRA; BATIZ, LUIS FEDERICO
Lugar:
MENDOZA
Reunión:
Congreso; XLVIII Reunión Anual de la Sociedad de Investigación en Bioquímica y Biología Molecular; 2012
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Several studies suggest that N-cadherin-based adherens junctions between granulosa cells (GC) play a key role in the ovarian follicle development. -SNAP, a protein involved in membrane fusion, has also been associated to the maintenance of cadherin-based junctions. We hypothesized that -SNAP is a regulator of Ncadherin- based cell-cell junctions between granulosa cells and follicle development. To test this we used a spontaneous mutant mouse model for -SNAP known as hyh (hydrocephalus with hop gait).We analyzed the expression of -SNAP in wild type (wt) and hyh ovaries from pre-pubertal to post-pubertal stages. Western blots showed that in wt, -SNAP increased at post-pubertal ages and after gonadotrophic stimulation; this increase correlates with the number of maturing follicles. On the other hand, hyh mice responded to hormonal stimulation, however, they showed lower levels of -SNAP at all stages, did not display the post-pubertal increase, and the number of maturing follicles was diminished. Immunofluorescence revealed that wt GC were joined together by well-developed N-cadherin-based adherens junctions; in contrast, hyh GC showed scarce adherens junctions and a punctate cytoplasmic pattern. Several GC with piknotic nuclei were observed in the follicular antrum. Our results suggest that -SNAP is important for the physiology of GC during follicle development in mouse.