IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Characterization of the relationship between NEI, TH and GAD-67 in hypothalamic brain areas of hypo- and hyperthyroid adult male rats
Autor/es:
CAROLINA AYALA; VALDEZ SUSANA; SOAJE MARTA; LOPEZ-AGUILERA FRANCISCO; CELIS, MARÍA ESTER; SELTZER, AM
Lugar:
Huerta Grande. Córdoba. Argentina
Reunión:
Congreso; XXVII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; 2012
Institución organizadora:
Sociedad Argentina en Neurociencias
Resumen:
Neuropeptide glutamic-acid-isoleucine amide (NEI) is a 13th aminoacid peptide derived from pp-MCH. Hypo- and hyperthyroidism modify NEI concentration in discrete brain areas of male [Ayala C et al. 2011] and female rats. The aim of the present study is to characterize the relationship between NEI-ir cells and fibers with tyrosine hidroxilase (TH) and acid-glutamic-descarboxilase-67 (GAD-67) in hypothalamic brain areas of hypo- and hyperthyroid adult male rats by means of immunofluorescence and confocal microscopy. Animals were divided into control, hypoT and hyperT (n=3 per group) and perfused 21 days after starting the treatment. The areas under study are the incerto-hypothalamic area (IHy), the peduncular part of the lateral hypothalamus (PLH) and the perifornix (PeF). Images are processed with Image J software. In the IHy at – 1.92 mm distance from Bregma, a 3D reconstruction was used to analyze the relationship between NEI and TH somas and dendritic tree. The optical density, cell area, the number of terminals and contact sites are quantified for each brain area and treatment. Differences among experimental groups are expected to be found according to preliminary observations.