IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
SERPIN 1F: DETECTION ON RAT TISSUES BY RT-PCR
Autor/es:
MONCLUS MARIA.; SAEZ LANCELLOTTI TANIA ESTEFANÍA; CAYADO NIUBYS ; PIETROBON ELISA; VINCENTI AMANDA EDITH; FORNES MIGUEL
Reunión:
Congreso; Reunión científica anual de la Sociedad de Biología de Cuyo.; 2012
Resumen:
In many mammals species during their epididymal journey, sperm
associated by their heads when they reach the Cauda, but not in the
proximal regions of this organ. These associations, called Rosettes
were previously characterized in Mouse and Rat. This suggests a
maturational process, probably due to the milieu that contains several
proteins secreted by the caudal epithelium.
The physiological meaning of this behavior remains unclear.
By chromatographic techniques we previously isolate and identified
SERPIN 1F, a member of the Serpins family (Serin proteases
inhibitors). This protein described by the first time in rat was involved
in the sperm re association during in vitro assays.
We isolate RNA from different epididymal regions and by RT-PCR
the presence of the Serpin 1F mRNA was observed in the samples
analyzed.