IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Study of the internalization pathway involved in Infectious Bursal Disease Virus infection
Autor/es:
M.C. GIMÉNEZ; J.F. RODRÍGUEZ; M.I. COLOMBO; L.R. DELGUI
Lugar:
Mendoza
Reunión:
Congreso; XLVII Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular. Mendoza; 2012
Resumen:
Infectious Bursal Disease Virus (IBDV) is an important avian pathogen member of the Birnaviridae family whose genome is composed of dsRNA. With the aim of analyzing the entry pathway involved in the viral infection, we studied the possible role of endocytosis in this process. Employing Laser Scanning Confocal Microscopy and Western blotting analysis we have observed an impaired IBDV infection of susceptible cells treated with endosomal acidification inhibitors such as BafilomycinA1 and ammonium chloride. In addition, overexpression of endosomal Rab proteins (i.e. Rab5 and Rab7), wild type or mutants, allowed us to demonstrate that the viral infection trafficking occurs along early and mature endosomes. Viral infection was not blocked by Dynasore, a dynamin-dependent endosome-scission inhibitor or by the depletion of membrane cholesterol by treatment with Methyl-β-Cyclodextrin of Filipin II, which remove cholesterol from cellular membranes. Ultrastructural analysis by Cryoelectron Microscopy of infected cells, analyzed at early times post infection, revealed the presence of viral particles attached to the cell membrane and within vesicles close to the membranes. Thus, we conclude that the early capture of virus into intracellular compartments is mediated by endosomes in a dynamin and cholesterol-independent fashion.