IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
DEHYDROLEUCODINE AFFECTS THE ORGANIZATION OF THE CYTOSKELETON AND THE FOCAL ADHESIONS OF HELA CELLS
Autor/es:
AMAYA MC, COSTANTINO VV, LOSINNO AD, BERTOLDI MV, LÓPEZ LA.
Lugar:
San Luis
Reunión:
Congreso; 47 th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2011
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Metastasis is the main cause of death in patients with cancer, therefore, it is relevant to find drugs to attack the cell invasion. Previously, we have demonstrated that Dehydroleucodine (DhL) has an inhibitory effect in the proliferation of HeLa cells. In this work we studied the effect of DhL on the migration of HeLa cells. Wound healing assay was performed in HeLa cells treated with 0 and 20μM DhL. We measured the speed and direction of migration and analyzed the cytoskeleton by immunofluorescence. DhL decreased the migration velocity (40%) and changed the dynamics of the advancing front of the cells. While control cells generated large and wide lamellipodia and migrated perpendicularly to the wound, DhL treated cells generated small lamellipodia and migrated erratically. Also, DhL treatment changed the organization of the cytoskeleton. In control cells the microtubules were concentrated in the central region and actin filaments formed an extensive network in the lamellipodia. In DhL treated cells, the microtubules were uniformly distributed in the cytoplasm and the actin filaments were packed in a thin line in the cell periphery. These results indicate that DhL affects the migration and the cytoskeleton of HeLa cells. DhL would be a potential agent to stop the invasion of tumor cells in the body.