IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Mast Cell Setting and differentiation in Lingual Tissues during Embrionyc Development and in New Born Rats
Autor/es:
ZAVALA W, FOSCOLO M, DAVILA J, CAVICCHIA, J.C
Lugar:
MERICA
Reunión:
Congreso; 11th Interamerican Congress of Electron Microscopy; 2011
Institución organizadora:
INTERAMERICAN SOCIETY OF MICROSCOPY
Resumen:
              Mast Cells (MC) connective tissue cells preferably found in immunological response areas such as in skin and mucosas [1]. These cells are activated by specific stimuli and release vasoactive amines, enzymes or differentiation mediators such as  the TNF (Tumour Necrotic Factor). In the oral mucosa, their function is still not well known. Some authors are of the opinion that they are involved in physiological processes like the cutaneous wound healing [2] or pathological ones like the oral lichen planus [3], gingivitis, periapical processes and in the immune response to diverse oral tumours [4,5,6]. Taking into consideration the significant role of MC in the mucosal immune system, the aim of the present study was to describe the morphology and to establish the periods of MC setting in lingual tissues during  embryonic development and in new born rats [7].  Rats of the Wistar strain were used in this study. Lingual fragments at 13,15,17,19 and 20 post copulation days were processed. Animals from firth day until the 14th post natal day were used in the post natal period. The material was fixed in paraformaldehyde and processed for optical, transmission electron microscopy and immunohistochemistry using specific monoclonal antibody against MC granules (antitriptase, clone AA1, from Imgenex).  Results slow round, small (7 ¡À 2mm) MC during embryonic development. They possess a small number of granules that progressively increase in number. After birth we observe a further progressive MC increase (the size increases to 14 ¡À 2 mm). Comparing the MC number between the first with the second week, we observe a significant increase (p ¡Ý0.001). After that, the cell number and morphology were similar to the adult rat.  Our results suggest an early migration of MC towards the embryonic lingual tissues and a fast adaptation to their new setting. This strategic distribution might permit them to respond to a variety of antigens that constantly are introduced in the oral cavity. Further studies will determinate more details about the presence and notable development of MC in the oral mucosa.