Antioxidant Defenses in an ischemia/reperfusion model induced by dehydration

CALDERÓN ML, GIRAUD-BILLOUD M, VEGA IA, ABUD MA, CASTRO- VÁZQUEZ A.

San Juan

Congreso; Segunda Reunión Conjunta de las Sociedades de Biología de la República Argentina; 2011

Pomacea canaliculata is an invasive gastropod that has been expanded to Asia, Europe and Hawaii were is an agriculture plague and an intermediate host for the agent of meningoencephalitis. This snail could survive longer periods of desiccation when the water is scarce, changing to an anaerobic metabolism (estivation) and have a specialized tissue that accumulates uric acid and this compound act as an antioxidant during reoxigenation occurred in the arousal of estivation. We quantificate the lipid peroxidation (TBARs), reduced glutathione (GSH), uric acid (UA), allantoin and protein concentrations and the activity of superoxide dismutase (SOD) and catalase (CAT) enzymes in kidney, midgut gland and foot muscle in control, post 45 days of estivation, 20 min or 24 h after reoxigenation during arousal from estivation groups. Kidney and foot had an increase in TBARs concentration after estivation and during arousal decrease. These levels didn´t change in the midgut gland. UA concentration decrease after arousal from estivation suggesting consume during their antioxidant action. All the organs count with the antioxidant action of GSH, since only in the midgut gland an increase in the CAT activity was observed. SOD activity was constant in the studied tissues of all groups. This work confirm our previous studies related to the antioxidant role of UA, observing that GSH contribute significantly to antioxidant defenses during the arousal from estivation and CAT is added to this defenses in midgut gland and kidney.