DEHYDROLEUCODINE DELAYS HeLa CELLS PROLIFERATION IN G1 OF THE NEW CELL CYCLE.

COSTANTINO VV, AMAYA MC, BERTOLDI MV, LOSINNO AD AND LÓPEZ LA.

San Juan

Congreso; Reunión Científica de las Sociedades de Biología de la República Argentina.; 2011

The Cancer is a disease characterized by the existence of an abnormal proliferation of cells. There are a lot of efforts in find agents that can block the uncontrolled cellular proliferation. The lactones sesquiterpene (SL) are the active components from medicinal plants and present a wide spectrum of biological activity. Dehydroleucodine (DhL) is a SL isolated from Artemisia douglasiana Besser, a plant that grew in the west Argentine region. In this work we analyzed the effect of DhL in the proliferation events of HeLa cells. Cells synchronized in G1 / S by double thymidine treatment, were stimulated with 10% fetal bovine serum (FBS) in presence of 0-30 µM DhL for 24 -72 h. We determined the growth rate (GR ± SEM) every 24 h and analyzed the cell distribution in G1,S and G2/M by flow cytometry analyses. At 72 h of culture, cells treated only with the vehicle (0 µM DhL) showed a GR of 5,611 ± 0,288, with 5 µM DhL 3.832 ± 0.297, with 10 µM DhL 2.649 ± 0.378, with 20 µM 1.735 ± 0.079, and with 30 µM 0.676 ± 0.048. The flow cytometry analyses indicated that 5 µM DhL arrested the cells in G2 / M for 8 h. However 20μM DhL arrested the cells in S phase 12 h, in G2 / M 4 h and permanently in G1 of the new cycle. These results indicate that DhL inhibits the proliferation of HeLa cells in a dose-dependent way arresting the cells transiently in S and G2/M and permanently in G1 of the new cycle.