IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ODF1, LABELLED BY MONOBROMOBIMANE, IS A SPERM FLAGELAR PROTEIN, ALSO LOCALIZED IN KIDNEY MEDULLA
Autor/es:
CABRILLANA ME; ANDERSEN ANA; IGLESIAS G; MONCLUS M. DE LOS A; SAEZ LANCELOTTI TS; BOARELLI PV; VICENTI AE; FORNES WM
Lugar:
San Juan
Reunión:
Congreso; 2da Reunión Conjunta de Sociedades de Biología de la República Argentina; 2011
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
ODF1, LABELLED BY MONOBROMOBIMANE, IS A SPERM FLAGELAR PROTEIN, ALSO LOCALIZED IN KIDNEY MEDULLA.   Cabrillana Me, Andersen A, Iglesias G, Monclus M. De Los A, Saez Lancelotti Ts, Boarelli Pv, Vincenti Ae, Fornés Mw.     Mammalian sperm proteins undergo thiol groups oxidation to form disulfides bonds as they travel through the epididymis during cell maturation. This proteins are involved in motility, sperm capacitation and acrosomal reaction. Our objectives were to determinate which proteins oxidate during epididymal transit, their physiology and if they are present in other tissue. In this work, we used a fluorescent thiol-selective labeling agent, monobromobimane, to study the protein thiol status of caput and cauda rat sperm. Fluorescence signal decreased along the epididymal trip, in sperm head and tail, indicating that both subcellular regions participate in the thiol changes. These sperm proteins also became resistant to be solubilized by SDS. Protein identification by mass spectrometry and sequence database searching correlated this protein with the ODF1. mBBr specifically bound to N-terminal domanin cysteine of ODF1. mBBr reduce rat and human sperm motility, quantitatively and qualitatively, and the effects are dose dependent, without significal increasing the percentage of dead sperm. Finaly, an ODF1 similar peptide were found in kidney, by western blotting techniques.