EFFECT OF PRENATAL TREATMENT WITH D-AMPHETAMINE ON THE TESTIS OF MALE ADULT RATS

PIETROBON ELISA OLIVIA; ROSALES J; GOMEZ S; MONCLUS MARÍA DE LOS ÁNGELES; MICHEL C; MACKERN-OBERTI JP; BREGONZIO C; SANCHEZ MB; NEYRA F; VALDEZ SR; SOAJE. M

Congreso; XL Reunion Anual de la Sociedad de Biologia de Cuyo; 2022

Prenatal amphetamine exposure (PEA) induces long-lasting changes that are evident even in adulthood. D-amphetamine (AMPH) is astimulant of CNS, it reverses the action of monoamine transporters, blocks the reuptake and degradation of dopamine (DA) andnoradrenaline (NA) increasing their availability. Evidence indicates that DA and NA participate in the regulation of gonadotrophin releasinghormone (GnRH) neurons during development and migration. Also, it has been demonstrated that the intake of AMPH derivatives inadulthood alters the hypothalamic-pituitary-gonadal axis, producing low levels of testosterone and poor sperm quality. The aim of the presentstudy was to evaluate the effects of prenatal AMPH exposure on testis during adulthood, particularly on the development of the seminiferousepithelium structure. Female rats were treated daily with AMPH 2.5mg/kg i.p or saline (SAL) during days 15 to 21 of pregnancy. On dayspost-natal 75-90, adult PEA and SAL treated male rats, were sacrificed by decapitation and the testis and epididymides were surgicallyremoved and weighed. Blood samples were obtained for testosterone determination by chemiluminescence method. Testes were fixed forthe histological analysis and the epididymides were sectioned and incubated in PBS at 37°C for the release and posterior sperm count. Next,they were prepared in HM (no capacitating medium) and HMB (capacitating medium) for the later analysis of the acrosomal reaction. Datawere analyzed using two-way ANOVA and Student´s-t test. No difference in weight testes was observed in PEA rats compared with SALtreated rats. Serum testosterone levels diminished significantly in PEA rats (p