Fractions of a chloroform extract of ajenjo leaves (Artemisia mendozana DC. var. mendozana) inhibit the proliferation, viability and clonogenicity of B16-F0 melanoma cells

MARRA, MARÍA FERNANDA; LOPEZ, LUIS A.; SALVARREDI, LEONARDO; MILLÁN, MARÍA E.; LIZARRAGA, EMILIO

BIOCELL

Tech Science Press

Lugar: Mendoza; Año: 2020 p. 1 - 7

1667-5746

Abstract: The ajenjo, Artemisia mendozana DC. var. mendozana (Asteraceae), grows in the Andean foothills of Mendozaand San Juan, Argentina, and is used as a medicinal plant for its antispasmodic and antifungal properties. The aim of thiswork was to obtain fractions of a chloroform extract of ajenjo leaves and to evaluate the in vitro effects on proliferation,viability and clonogenicity of B16-F0 melanoma cells. Using a silica gel chromatography column, 120 fractions werecollected and grouped according to the chromatographic profile in 9 main fractions (F1?F9). Their major compoundsidentified were: terpenes (F1), terpenes and sesquiterpene lactones (F2?F3), sesquiterpenes (F4?F6) and phenols andsesquiterpenes (F7-9). B16-F0 cells were incubated for 72 h with DMSO (vehicle) or 0.1 mg/ml F1?F9. At 72 h ofculture, F1 decreased both the growing index (GI) and cell viability. F2 and F3 both decreased GI and only F3decreased clonogenic activity. F4 and F5 both decreased GI. Only F5 decreased cell viability and F4 decreasedclonogenicity. Consequently, fractions F6?F8 did not affect any of the cell parameters assayed, while F9 decreased cellviability and inhibited clonogenicity