IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
artículos
Título:
Semen processing and sperm function: Differences between swim-up and density gradient centrifugation
Autor/es:
GABRIELA HERNÁNDEZ-SILVA; ESPERANZA MATA MARTÍNEZ; MAYEL CHIRINOS; AIDEÉ S. LÓPEZ-TORRES; FERNANDO LARREA; ISRAEL MALDONADO; VÍCTOR TORRES-FLORES
Revista:
The World Journal of Men's Health
Editorial:
Korean Society for Sexual Medicine and Andrology
Referencias:
Lugar: Korea; Año: 2020
ISSN:
2287-4208
Resumen:
Andrology research has evolved notoriously in the latest years, mostly since male factor contribution to couple infertility problems has been undoubtedly demonstrated. However, data from basic and clinic investigations sometimes seem contradictory, probably as a result of different sperm processing techniques. In this work we underwent a systematic comparison of human sperm samples processed by swim-up and density gradient centrifugation, which are the preferred sperm processing methods used basic and clinical laboratories, respectively. Methods: Comparative analysis of the effects of swim-up and density gradient centrifugation followed by incubation under capacitating conditions for 0, 2, 4 and 6 h on sperm redox state, intracellular calcium oscillations, hyperactivation, protein tyrosine phosphorylation and acrosome reaction responsivity to calcium ionophore. Results: Semen samples simultaneously processed by these two procedures showed significant differences in all sperm variables analyzed. Remarkably, differences in protein tyrosine phosphorylation were noticeable right after sperm processing but tend to disappear at long incubations times, while differences in sperm hyperactivation increased with time. Conclusions: Results can account for discrepancies on sperm differences obtained with these two methods and therefore may contribute to a better interpretation of the results obtained in andrology laboratories as well as assist to overcome difficulties to perform translational investigations in this field.