Freely Available Tool (FAT) for automated quantification of lipid droplets in stained cells

ALDANA GOJANOVICH; MARINA UHART; DIEGO MASONE; ALDANA GOJANOVICH; SAMANTA DEL VÉLIZ; YESICA R. FRONTINI LÓPEZ; MARINA UHART; DIEGO MASONE; DIEGO M. BUSTOS; SAMANTA DEL VÉLIZ; YESICA R. FRONTINI LÓPEZ; DIEGO M. BUSTOS

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2017 vol. 41 p. 55 - 58

0327-9545

In this study, wepropose an automatic procedure for digital image processing. Wedescribe a method that can efficiently quantify and characterizelipid droplets distributions in different cell types in culture.Prospectively, the lipid droplets detection method described in thiswork could be applied to static or time-lapse data, collected with asimple visible light or fluorescence microscopy equipment. Fullyautomated algorithms were implemented in Octave, a freely availablescientific package.p { margin-bottom: 0.25cm; line-height: 115%; }