Antiproliferative effect of essential oil of Artemisia mendozana in tumor cells B16F0.

DARIO FERNANDEZ; LUIS A LOPEZ; ELISA MILLAN ; EMILIO LIZARRAGA

MEDICINA (BUENOS AIRES)

MEDICINA (BUENOS AIRES)

Lugar: Buenos Aires; Año: 2017 vol. 77

0025-7680

ANTIPROLIFERATIVE EFFECT OF ESSENTIAL OIL OF Artemisia mendozana IN TUMOR CELLS B16F0.Millán M E, Lizarraga E, Fernandez D, Lopez L A. IHEM CCT-Conicet Mendoza, and Institute of Animal Physiology, Miguel Lillo Foundation, San Miguel de Tucumán. E-mail: llopez@fcm.edu.arThe basic pathophysiology of cancer comprises aberrations at different points in the cell cycle. Due to the increasing incidence of cancer worldwide, there is an intense search for new therapeutic strategies to treat this disease. In this area, important efforts have been oriented to exploring the action of compounds of plant origin. Artemisia mendozana var mendozana (ajenjo), is a plant belonging to the Asteraceae family that growth in the piedmont of Mendoza province and it is used as a medicinal plant with antispasmodic and antifungal properties. The essential oil of ajenjo (EO) contains 28 compounds of which the higher concentrated are: artemisia alcohol 4.8%, α-thujone 5.1%, borneol 11.2% and bornyl acetate 43.7%. In this project the effect of EO was analyzed in the in vitro proliferation of B16F0 murine melanoma cells. For the assay the cells were cultured with vehicle DMSO (control) or 0.1- 0.25 μl / mL EO dissolved in DMSO for 72 h. The growth rate (GI) ± SE was calculated from 3 independent experiments. At 72 h of culture, the GI of the control was 6.37 ± 1.07 and with EO was: 4.57 ± 1.07 (A); 1.37 ± 0.18 (B) and 0.43 ± 0.03 (C) for 0.13, 0.2 and 0.25 μL / mL respectively. GI of B and C were significantly different to the control (p≤0.001). These results show that EO, at concentrations, is a powerful inhibitor of the in vitro proliferation of B16 F0 cells. General, Cellular and Molecular Biology (BM).