CHANGES IN RENAL WT-1 EXPRESSION PRECEDING HYPERTENSION DEVELOPMENT

ISABEL MERCEDES GARCÍA; MIGUEL FORNÉS; WALTER MANUCHA; LUCIANA MAZZEI; MARIANA CASAROTTO; LEÓN FERDER; JUAN PABLO CALVO; DARÍO CUELLO-CARRIÓN

BMC Nephrology

Springer

Lugar: Londres; Año: 2016 vol. 17 p. 1 - 14

Hypertension is a public health problem mostly unknown causes, and where strong hereditary genetic alterations, have not been fully elucidated. However, the use of experimental models, have provided valuable information. Interestingly, recent evidences suggest that alterations in keys nephrogenic factors such Wilms tumor 1 factors (WT-1), could contribute to the development of hypertension. Aims: To evaluate the expression of WT-1 and related genes in the nephrogenic process associated with the development of hypertension as well as the corresponding anatomical and functional correlation. Male spontaneously hypertensive rats (SHR) were evaluated weekly from birth until the eighth week of life. We established the relationship of renal weight/total body weight without values significantly different. These data were compared with apoptosis, fibrosis, number and size of the glomeruli. The elevation of systolic blood pressure was significant since the sixth week. Biochemical values were slightly modified. Histology showed an increase in deposits of collagen fibers since the 4th week. Additionally, decreased expression of WT-1, heat shock protein 70 (Hsp70) and vitamin D receptors (VDR) since the fourth week in SHR were shown. Finally, we demonstrated ultra-structural damage to mitochondria since the fourth week of life. Our results would suggest an unprecedented link, possibly regulatory mechanistic, of WT-1 on nephrogenic alteration processes and their relationship with hypertension. If so, it could suggest that deregulation in the expression of WT-1 and its impact on the nephrogenesis induction; they could be crucial in understanding the development and maintenance of hypertension.