IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
artículos
Título:
Advancement of reproductive senescence and changes in the early expression of oestrogen,
Autor/es:
EZQUER ME; VALDEZ SR,; SELTZER A.M.,; JAHN GA
Revista:
BRAIN RESEARCH
Editorial:
ELSEVIER
Referencias:
Año: 2008
ISSN:
0006-8993
Resumen:
Abstract: Abstract
Perinatal hypoxia is a frequent birth complication, and although its early consequences on brain
development have been well studied, few studies address any long-term effects. Postnatal insults producing
small disturbances in endocrine function can have marked and long-lasting effects. In the present work we
studied the effects of two types of perinatal brain injury: global hypoxia (H, 6.5 % O2 for 50 min) and hypoxia
plus ischemia (HI, ligature of the right carotid artery) on female rat reproductive performance and expression
of mediobasal hypothalamus-preoptic area (MBH-PO) estrogen, progesterone and £g opioid receptors at
different times after injury, measuring the mRNA (by semiquantitative RT-PCR) and protein (by Western
blot). H or HI advanced approximately 3 months the appearance of blunted preovulatory LH surges and
cyclic irregularities (prolonged estrus) characteristic of the early stages of reproductive senescence. 48 h
after H or HI we observed decreases in ER£]zn£gOR and PR (only in the H group) mRNAs and in total ER and
£gOR proteins, followed by increased PR levels (mRNA and protein) 7 days post-injury and by increased
£gOR protein and ER£] mRNA in the H group and ER£, ER£]n and n£gOR mRNAs and ER protein in the HI
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
£gOR proteins, followed by increased PR levels (mRNA and protein) 7 days post-injury and by increased
£gOR protein and ER£] mRNA in the H group and ER£, ER£]n and n£gOR mRNAs and ER protein in the HI
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
£gOR proteins, followed by increased PR levels (mRNA and protein) 7 days post-injury and by increased
£gOR protein and ER£] mRNA in the H group and ER£, ER£]n and n£gOR mRNAs and ER protein in the HI
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
£gOR proteins, followed by increased PR levels (mRNA and protein) 7 days post-injury and by increased
£gOR protein and ER£] mRNA in the H group and ER£, ER£]n and n£gOR mRNAs and ER protein in the HI
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
zn£gOR and PR (only in the H group) mRNAs and in total ER and
£gOR proteins, followed by increased PR levels (mRNA and protein) 7 days post-injury and by increased
£gOR protein and ER£] mRNA in the H group and ER£, ER£]n and n£gOR mRNAs and ER protein in the HI
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.
n and n£gOR mRNAs and ER protein in the HI
group30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature
reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone
receptors (£gOR, ER and PR), whose expression is intimately involved in the regulation of gonadotrophin
secretion and female sexual cyclicity.