NONLINEAR CONTROL OF THE DISSOLVED OXYGEN CONCENTRATION FOR OPTIMAL PRODUCTION OF BACILLUS THURINGIENSIS δ-ENDOTOXINS

ROMOLI SANTIAGO; AMICARELLI, A; OSCAR ORTIZ; JORGE R.VEGA; DI SCIASCIO FERNANDO; ABALLAY PABLO; SCAGLIA GUSTAVO

Buenos Aires

Congreso; VIII Congreso Argentino de Ingeniería Química - CAIQ2015; 2015

Bacillus thuringiensis is a microorganism that allows the biosynthesis of δ-endotoxins with toxic properties against some insect larvae, being often used for the production of biological insecticides. A key issue for the bioprocess design consists in adequately tracking a pre-specified optimal profile of the dissolved oxygen concentration. To this effect, this paper aims at developing a novel control law based on a nonlinear dynamic inversion method. The closed-loop strategy includes an observer based on a Bayesian Regression with Gaussian Process, which is used for on-line estimating the biomass present in the bioreactor. Unlike other approaches, the proposed controller leads to an improved response time with effective disturbance rejection properties, while simultaneously prevents undesired oscillations of the dissolved oxygen concentration. Simulation results based on available experimental data were used to show the effectiveness of the proposal.